Format

Send to

Choose Destination
See comment in PubMed Commons below
Cell Cycle. 2006 Oct;5(19):2260-7. Epub 2006 Oct 1.

Condensin function in mitotic nucleolar segregation is regulated by rDNA transcription.

Author information

  • 1NICHD, Laboratory of Gene Regulation and Development, Bethesda, Maryland 20892, USA.

Abstract

Chromosome condensation is established and maintained by the condensin complex. The mechanisms governing loading of condensin onto specific chromosomal sites remain unknown. To elucidate the molecular pathways that determine condensin binding to the nucleolar organizer, a key condensin binding site, we analyzed the properties of condensin-bound sites within the rDNA repeat in budding yeast and demonstrated that the bulk of mitotic condensin binding to rDNA is reduced or eliminated when Pol I transcription is elevated. Conversely, when Pol I transcription is repressed either by rapamycin treatment or by promoter shut-off, condensin binding to rDNA is increased. This novel potential role for constitutive and/or periodic repression of Pol I transcription in rDNA condensin loading is an important factor in determining the segregation proficiency of NOR-containing chromosomes.

PMID:
16969110
PMCID:
PMC3225123
DOI:
10.4161/cc.5.19.3292
[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Taylor & Francis Icon for PubMed Central
    Loading ...
    Support Center