Real-time PCR TaqMan assay for detecting Trichophyton tonsurans, a causative agent of tinea capitis, from hairbrushes

T Sugita; Y Shiraki; M Hiruma

doi:10.1080/13693780600717153

Real-time PCR TaqMan assay for detecting Trichophyton tonsurans, a causative agent of tinea capitis, from hairbrushes

Med Mycol. 2006 Sep;44(6):579-81. doi: 10.1080/13693780600717153.

Authors

T Sugita¹, Y Shiraki, M Hiruma

Affiliation

¹ Department of Microbiology, Meiji Pharmaceutical University, Kiyose, Japan. sugita@my-pharm.ac.jp

PMID: 16966179
DOI: 10.1080/13693780600717153

Abstract

Tinea capitis caused by Trichophyton tonsurans is currently an epidemic in the United States, Europe, and Japan, and the cultivation of this microorganism is necessary for a definitive diagnosis. We recently developed a real-time PCR TaqMan assay as a culture-independent method for the rapid detection of T. tonsurans from hairbrushes.

MeSH terms

Humans
Polymerase Chain Reaction / methods*
Taq Polymerase / metabolism
Tinea Capitis / diagnosis
Tinea Capitis / epidemiology
Tinea Capitis / microbiology*
Trichophyton / classification
Trichophyton / growth & development
Trichophyton / isolation & purification*

Substances

Taq Polymerase