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Fertil Steril. 2006 Oct;86(4):997-1000. Epub 2006 Sep 11.

Ongoing pregnancies after vitrification of human oocytes using a combined solution of ethylene glycol and dimethyl sulfoxide.

Author information

1
Centro Sterilità e Fecondazione Assistita Policlinico di Perugia, University of Perugia, Perugia, Italy. selmanha@yahoo.com

Abstract

OBJECTIVE:

To evaluate a vitrification solution using a mixture of two cryprotectant agents, dimethyl sulfoxide and ethylyne glycol plus sucrose, on the survival of human oocytes.

DESIGN:

Clinical study of cryopreservation of human metaphase II (MII) oocytes by vitrification.

SETTING:

University-affiliated IVF center.

PATIENT(S):

Infertile couples who agreed to have their surplus oocytes vitrified during the fresh IVF cycle.

INTERVENTION(S):

Vitrification of surplus oocytes subsequently used in the next cycle and assisted fertilization by intracytoplasmic sperm injection.

MAIN OUTCOME MEASURE(S):

Morphologic survival and normal fertilization, embryo development, and clinical outcome.

RESULT(S):

A total of 53 surplus MII oocytes from 6 patients were vitrified, of which 24 were thawed, resulting in 18 which survived morphologically (75%). Following insemination, 14 of the 18 surviving eggs were fertilized (77.7%). All zygotes developed into viable embryos that were replaced into each patient's uterus, resulting in two healthy pregnancies: one singleton and one twin. The pregnancies were ongoing.

CONCLUSION(S):

Cryopreservation of human MII oocytes by vitrification appears to be a promising procedure, though to assure optimal effectiveness of this protocol further studies should be undertaken.

[Indexed for MEDLINE]

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