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Science. 2006 Sep 8;313(5792):1441-3.

Humanization of yeast to produce complex terminally sialylated glycoproteins.

Author information

  • 1GlycoFi Inc., 21 Lafayette Street, Suite 200, Lebanon, NH 03766, USA.

Abstract

Yeast is a widely used recombinant protein expression system. We expanded its utility by engineering the yeast Pichia pastoris to secrete human glycoproteins with fully complex terminally sialylated N-glycans. After the knockout of four genes to eliminate yeast-specific glycosylation, we introduced 14 heterologous genes, allowing us to replicate the sequential steps of human glycosylation. The reported cell lines produce complex glycoproteins with greater than 90% terminal sialylation. Finally, to demonstrate the utility of these yeast strains, functional recombinant erythropoietin was produced.

PMID:
16960007
DOI:
10.1126/science.1130256
[PubMed - indexed for MEDLINE]
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