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Methods Mol Biol. 2006;342:49-56.

Recognition and cleavage of primary microRNA transcripts.

Author information

1
Department of Pharmacology, University of Minnesota, Minneapolis, MN, USA.

Abstract

MicroRNAs (miRNAs) are approx 22-nucleotide (nt)-long, single-stranded, endogenous, noncoding RNAs that are widely expressed in multicellular organisms. This chapter describes methods that allow the overexpression of human miRNAs and also discusses how primary miRNAs (pri-miRNAs), the much longer precursors of mature miRNAs, are processed in human cells, as well as in vitro.

PMID:
16957366
DOI:
10.1385/1-59745-123-1:49
[Indexed for MEDLINE]

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