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Micron. 2007;38(2):150-7. Epub 2006 Jul 31.

Coherent super-resolution microscopy via laterally structured illumination.

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1
Centre for Biophotonics and Laser Science, University of Queensland, St. Lucia 4072, Australia. littleton@physics.uq.edu.au

Abstract

Theory describing a super-resolution microscopy experiment using temporally and spatially coherent structured illumination was developed, and used to derive a method for processing experimental data. Numerical simulations were performed to verify that the method can, in principle, produce super-resolved images that are exactly equivalent to an image processed by a system with a much larger aperture (that is, the correct weighting between different regions of the image spectrum is maintained). The process was then demonstrated experimentally, showing a factor of two improvement in resolution over a diffraction-limited, coherently illuminated, microscope.

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