The aim of our study was to evaluate the use of the medical Internet by midwifery students with analysis of influencing factors and students' preferences. A prospective cross-sectional questionnaire study was carried out with 180 midwifery students. The response rate was 83.8%, 82% use the Internet, and 79.3% use the medical resources in their education. The mean accession to the Web is 5.3 +/- 8.4 times per month. The mean evaluation of information resources for books, journals, conferences and university courses was 6.6 +/- 3.2, 4.8 +/- 3.1, 2.7 +/- 3.0, and 6.8 +/- 3.8, respectively, as measured with the Visual Analogue Scale (p < 0.001). Age did not correlate with Internet use and its evaluation but correlated with a self-evaluation of Internet skills as a user (Rs = -0.20, p = 0.016). The evaluation of the Internet as a source of medical knowledge correlated with self-evaluation of medical search skills (Rs = 0.66, p < 0.001) and user skills (Rs = 0.61, p < 0.001). The medical Internet is used by the majority of midwifery students, but it is still a secondary source of information, after university training and medical books. The higher evaluation of Internet correlated with general computer skills and medical search skills, which suggests the need for education on Internet use by students.