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Microb Pathog. 2006 Dec;41(6):207-17. Epub 2006 Sep 6.

Expression of the Escherichia coli IrgA homolog adhesin is regulated by the ferric uptake regulation protein.

Author information

1
Department of Microbiology, University of Washington, 1959 NE Pacific St. Mailstop 357242, Seattle, WA 98195, USA. rrashid@alumni.indiana.edu <rrashid@alumni.indiana.edu>

Abstract

The IrgA homolog adhesin (Iha) is an adherence-conferring outer membrane protein of Escherichia coli associated with enterohemorrhagic and uropathogenic strains. Here, we used primer extension analysis to identify iha promoters in O157:H7 and uropathogenic E. coli strains. Transcriptional fusions demonstrated that iha transcription is repressed by iron. Gel shifts using purified ferric uptake regulator protein (Fur) demonstrated that repression involves a direct interaction between Fur and the iha promoter. We identified strain-dependent differences in iha expression and determined that single nucleotide polymorphisms upstream of the iha promoter, in particular position -85, contribute to differences in expression levels.

PMID:
16954050
PMCID:
PMC2039900
DOI:
10.1016/j.micpath.2006.07.006
[Indexed for MEDLINE]
Free PMC Article

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