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Microbes Infect. 2006 Sep;8(11):2587-98. Epub 2006 Aug 14.

High resolution radiographic and fine immunologic definition of TB disease progression in the rhesus macaque.

Author information

1
Pulmonary & CCM, R&D 11, Portland VA Medical Center, 3710 SW US Veterans Road, Portland, OR 97239, USA. lewinsod@ohsu.edu <lewinsod@ohsu.edu>

Abstract

Mycobacterium tuberculosis infection in non-human primates parallels human tuberculosis, and provides a valuable vaccine evaluation model. However, this model is limited by the availability of real-time, non-invasive information regarding disease progression. Consequently, we have combined computed tomography scanning with enumeration of antigen-specific T cell responses. Four rhesus monkeys were infected with M. tuberculosis strain H37Rv (1000 cfu) in the right lower lobe via a bronchoscope. All uniformly developed progressive tuberculosis, and required euthanasia at 12 weeks. Computed tomography scanning provided detailed real-time imaging of disease progression. At necropsy, computed tomography and pathohistologic findings were tightly correlated, and characteristic of human disease. Immunologic monitoring demonstrated progressive evolution of high frequency M. tuberculosis-specific CD4(+) and CD8(+) T cell responses. Peripheral blood effector cell frequencies were similar to those observed in tissues. In summary, computed tomography scanning in conjunction with immunologic monitoring provides a non-invasive, accurate, and rapid assessment of tuberculosis in the non-human primate.

PMID:
16952476
DOI:
10.1016/j.micinf.2006.07.007
[Indexed for MEDLINE]

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