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Genetics. 2006 Oct;174(2):1057-61. Epub 2006 Sep 1.

Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction.

Author information

1
Department of Plant Biology, University of Georgia, Georgia 30602, USA.

Abstract

Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.

PMID:
16951073
PMCID:
PMC1602074
DOI:
10.1534/genetics.106.060467
[Indexed for MEDLINE]
Free PMC Article

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