A fused gene of nucleoprotein (NP) and herpes simplex virus genes (VP22) induces highly protective immunity against different subtypes of influenza virus

Sukumar Saha; Shinsuke Yoshida; Kenji Ohba; Kiyohiko Matsui; Tomoko Matsuda; Fumihiko Takeshita; Kazunori Umeda; Yuichi Tamura; Kentaro Okuda; Dennis Klinman; Ke-Qin Xin; Kenji Okuda

doi:10.1016/j.virol.2006.04.015

A fused gene of nucleoprotein (NP) and herpes simplex virus genes (VP22) induces highly protective immunity against different subtypes of influenza virus

Virology. 2006 Oct 10;354(1):48-57. doi: 10.1016/j.virol.2006.04.015. Epub 2006 Aug 30.

Authors

Sukumar Saha¹, Shinsuke Yoshida, Kenji Ohba, Kiyohiko Matsui, Tomoko Matsuda, Fumihiko Takeshita, Kazunori Umeda, Yuichi Tamura, Kentaro Okuda, Dennis Klinman, Ke-Qin Xin, Kenji Okuda

Affiliation

¹ Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Yokohama 236-0004, Japan.

PMID: 16945400
DOI: 10.1016/j.virol.2006.04.015

Abstract

We evaluated the immunogenicity and protective activity of plasmid DNA vaccines encoding the influenza virus NP gene (pNP) alone or in combination with the herpes simplex virus type 1 protein 22 gene (pVP22). Optimal immune responses were observed in BALB/c mice immunized with the combination of pVP22 plus pNP, as assessed by enzyme-linked immunosorbent assay (ELISA), enzyme-linked immunospot (ELISPOT) and intracellular cytokine staining (ICCS). These mice also showed maximal resistance following challenge with the A/PR/8/34 (H1N1) and A/Udron/72 (H3N2) strains of influenza virus. The susceptibility of immunized mice to virus infection was significantly increased following depletion of either CD4+ or CD8+ T cells. These results indicate that a plasmid DNA vaccine encoding pVP22 plus NP induces a high level of cross-protective immunity against influenza virus subtypes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Viral / blood
Artificial Gene Fusion
Body Weight
Cytokines / biosynthesis
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Genetic Vectors
Humans
Influenza A virus / immunology*
Influenza Vaccines / genetics
Influenza Vaccines / immunology*
Influenza, Human / immunology
Influenza, Human / prevention & control*
Lymphocyte Depletion
Lymphocyte Subsets / immunology
Mice
Mice, Inbred BALB C
Nucleocapsid Proteins
Nucleoproteins / genetics
Nucleoproteins / immunology*
Plasmids / genetics
RNA-Binding Proteins / genetics
RNA-Binding Proteins / immunology*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / immunology
T-Lymphocytes / immunology
Vaccines, DNA / genetics
Vaccines, DNA / immunology*
Viral Core Proteins / genetics
Viral Core Proteins / immunology*
Viral Structural Proteins / genetics
Viral Structural Proteins / immunology*

Substances

Antibodies, Viral
Cytokines
Influenza Vaccines
NP protein, Influenza A virus
Nucleocapsid Proteins
Nucleoproteins
RNA-Binding Proteins
Recombinant Fusion Proteins
Vaccines, DNA
Viral Core Proteins
Viral Structural Proteins
herpes simplex virus type 1 protein VP22