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Biol Cell. 1990;68(2):167-70.

In situ separation of nucleolar components by hypotonic treatment of cells.

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1
Institute of Experimental Medicine, Czechoslovak Academy of Sciences, Prague.

Abstract

The main features of the reaction of nucleolar components to a simple hypotonic shock are described. In place of nucleoli, only fibrillar complexes remain. Such preservation provides another morphological proof of the hypothesis of fibrillar complexes as morphological-functional units in active nucleoli. Moreover, this method can become a good tool for further study of nucleolus-organizing regions.

PMID:
1694098
[Indexed for MEDLINE]

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