Format

Send to

Choose Destination
Nat Chem Biol. 2006 Oct;2(10):529-30. Epub 2006 Aug 27.

Kinetic flux profiling of nitrogen assimilation in Escherichia coli.

Author information

1
Department of Chemistry and Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA.

Abstract

We present a new method for probing cellular metabolic fluxes that is based on the kinetics of assimilation of isotope-labeled nutrient into a diversity of downstream metabolites. In the case of nitrogen assimilation, half-maximal labeling of most metabolites occurs in 10-300 s. Fluxes measured on the basis of the kinetics of nitrogen assimilation in exponentially growing E. coli agree well with those fluxes predicted to allow optimal biomass production.

PMID:
16936719
DOI:
10.1038/nchembio816
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center