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J Am Soc Mass Spectrom. 2006 Dec;17(12):1700-3. Epub 2006 Aug 22.

Semi-automated data processing of hydrogen exchange mass spectra using HX-Express.

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Department of Chemistry, University of New Mexico, Albuquerque, New Mexico, USA.


A Microsoft Excel utility, HX-Express, that significantly accelerates the analysis of hydrogen exchange mass spectrometry data is described. HX-Express generates deuterium uptake and peak width plots from peaks in mass spectral data. Data analysis is intentionally semi-automated, requiring that the user find the peaks to be analyzed. The peaks are entered in the form of x, y lists of m/z versus intensity or can be directly imported from Waters MassLynx software. Analysis of data with HX-Express provides the same results as manual data processing but is substantially faster. In addition to speed, HX-Express provides and preserves visual and numeric displays of the analysis process for quality control.

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