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Methods Mol Biol. 2006;337:3-13.

Functional reconstitution of the human epithelial Na+ channel in a mammalian expression system.

Author information

1
Department of Physiology, University of Texas Health Science Center at San Antonio, USA.

Abstract

Probing ion channel structure-function and regulation in native tissue can, in some instances, be experimentally challenging or impractical. To facilitate discovery and increase experimental flexibility, our laboratory routinely reconstitutes recombinant ion channels in a mammalian expression system quantifying channel activity with patch clamp electrophysiology. Here, we describe investigation of the human epithelial Na+ channel heterologously expressed in Chinese hamster ovary cells.

PMID:
16929934
DOI:
10.1385/1-59745-095-2:3
[Indexed for MEDLINE]

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