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Biotechniques. 1990 Apr;8(4):357-60.

Stability of RNA isolated from macrophages depends on the removal of an RNA-degrading activity early in the extraction procedure.

Author information

1
Jackson Laboratory, Bar Harbor, ME 04609.

Erratum in

  • Biotechniques 1990 Jul;9(1):55.

Abstract

Ubiquitous RNases are the usual causes of RNA degradation on its isolation from mammalian cells. Using guanidine hydrochloride for the extraction of RNA from mouse peritoneal macrophages, we identify a major source of RNA-degrading activity, the stage of the extraction procedure at which this activity may be detected and show that its removal early in the extraction leads to a more dependable method for the recovery of high quality RNA.

PMID:
1692714
[Indexed for MEDLINE]

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