Encapsulation of single enzyme in nanogel with enhanced biocatalytic activity and stability

Ming Yan; Jun Ge; Zheng Liu; Pingkai Ouyang

doi:10.1021/ja064126t

Encapsulation of single enzyme in nanogel with enhanced biocatalytic activity and stability

J Am Chem Soc. 2006 Aug 30;128(34):11008-9. doi: 10.1021/ja064126t.

Authors

Ming Yan¹, Jun Ge, Zheng Liu, Pingkai Ouyang

Affiliation

¹ Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.

PMID: 16925402
DOI: 10.1021/ja064126t

Abstract

Single protein encapsulated into nanogels with uniformed size and controllable shell thickness were prepared by surface acryloylation of a protein molecule followed by aqueous in situ polymerization. Compared to its free counterpart, the encapsulated protein exhibits similar biocatalytic behavior and significantly improved stability at high temperature and in the presence of organic solvent.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Catalysis
Enzyme Stability
Gels*
Horseradish Peroxidase / metabolism*
Microscopy, Electron, Transmission
Nanostructures*

Substances

Gels
Horseradish Peroxidase