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Cell Tissue Res. 1990 Feb;259(2):199-207.

Synchronized endocytosis studied in the oocyte of a temperature-sensitive mutant of Drosophila melanogaster.

Author information

1
Division of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California 91010.

Abstract

This study demonstrates that endocytosis in the oocyte of Drosophila melanogaster is reversibly blocked at the stage of pit formation by the temperature-sensitive, single-gene mutant, shibirts1. Uptake of horeradish peroxidase conjugated with wheat-germ agglutinin was observed to be normal in mutant oocytes at 19 degrees C, but was blocked at 29 degrees C. After 10 min at 29 degrees C, there was a build-up of coated pits along invaginations of the plasma membrane. Also, the endosomal compartment consisting of tubules, bulbs, and small yolk spheres, disappeared. Lowering the temperature to 19 degrees C after 10 min at 29 degrees C released a synchronized wave of endocytosis into a cytoplasm cleared of uptake-related organelles. By observing this synchronized wave after exposure to 19 degrees C for varying durations, we determined that endocytosis proceeds as follows: coated pits/vesicles----tubules----small yolk spheres----mature yolk spheres. The observations suggest that these organelles transform one into another within this sequence.

PMID:
1692513
DOI:
10.1007/bf00318441
[Indexed for MEDLINE]

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