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Methods Enzymol. 1990;182:602-13.

Limited N-terminal sequence analysis.

Abstract

Current methods in protein purification permit sequence analysis of picomole quantities of proteins and peptides. The key to obtaining sequence is to choose as the last step in the purification protocol the simplest (usually the fastest) method for isolating the protein or peptide.

PMID:
1690332
DOI:
10.1016/0076-6879(90)82047-6
[Indexed for MEDLINE]

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