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Nat Methods. 2006 Sep;3(9):721-3.

Nanoscale resolution in GFP-based microscopy.

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1
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany.

Abstract

We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The approximately 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.

PMID:
16896340
DOI:
10.1038/nmeth922
[Indexed for MEDLINE]
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