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Nat Methods. 2006 Oct;3(10):793-5. Epub 2006 Aug 9.

Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).

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1
Department of Physics, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA.

Abstract

We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.

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PMID:
16896339
PMCID:
PMC2700296
DOI:
10.1038/nmeth929
[Indexed for MEDLINE]
Free PMC Article

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