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Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12400-4. Epub 2006 Aug 8.

West Nile virus in complex with the Fab fragment of a neutralizing monoclonal antibody.

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  • 1Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907-2054, USA.

Abstract

Flaviviruses, such as West Nile virus (WNV), are significant human pathogens. The humoral immune response plays an important role in the control of flavivirus infection and disease. The structure of WNV complexed with the Fab fragment of the strongly neutralizing mAb E16 was determined to 14.5-Angstrom resolution with cryo-electron microscopy. E16, an antibody with therapeutic potential, binds to domain III of the WNV envelope glycoprotein. Because of steric hindrance, Fab E16 binds to only 120 of the 180 possible binding sites on the viral surface. Fitting of the previously determined x-ray structure of the Fab-domain III complex into the cryo-electron microscopy density required a change of the elbow angle between the variable and constant domains of the Fab. The structure suggests that the E16 antibody neutralizes WNV by blocking the initial rearrangement of the E glycoprotein before fusion with a cellular membrane.

PMID:
16895988
PMCID:
PMC1567891
DOI:
10.1073/pnas.0603488103
[PubMed - indexed for MEDLINE]
Free PMC Article
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