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Biochem Biophys Res Commun. 2006 Sep 22;348(2):716-21. Epub 2006 Jul 31.

Subcellular dynamics of protein kinase A activity visualized by FRET-based reporters.

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1
Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Abstract

cAMP-dependent protein kinase (PKA) mediates key cellular processes via compartmentalized activity, and the ability to track its activity in living cells should help increase our understanding of this precise regulation. Here, through systematic testing of new fluorescent proteins, we developed a new FRET-based A-kinase activity reporter (AKAR), AKAR3, with a dynamic range of 31-41%, twice that of predecessors. Visualization of PKA activity at plasma membrane, cytoplasm, nucleus, and mitochondria was achieved. Targeting AKAR3 to outer mitochondrial membrane revealed that basal PKA activity at mitochondria differs from that in the cytoplasm, indicating differential regulation of PKA activity at different subcellular locations.

PMID:
16895723
DOI:
10.1016/j.bbrc.2006.07.136
[Indexed for MEDLINE]
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