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Biotechnol J. 2006 Jun;1(6):651-5.

A rapid, high content, in vivo model of glucocorticoid-induced osteoporosis.

Author information

1
DanioLabs Ltd, Cambridge, UK.

Abstract

Glucocorticoid-induced osteoporosis (GIOP) is a major clinical problem given the widespread use of steroids and limited efficacy of biphosphonates. Existing animal models of GIOP are both slow and expensive. Hence, there is a need both for adjunctive modelling systems, as well as more efficacious therapies for the treatment of GIOP. We have addressed this issue through the creation of a zebrafish model of GIOP, which can be used for 96-well plate in vivo screening with an assay time of 5 days. The model demonstrates key similarities to human GIOP including a partial response to bisphosphonates. The ability to extract detailed pharmacological data, including concentration-response analyses, enables the screening and ranking of candidate therapeutic compounds. In addition, the zebrafish model is highly relevant for pathway dissection through genetic knockdown and overexpression studies.

PMID:
16892313
DOI:
10.1002/biot.200600043
[Indexed for MEDLINE]

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