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J Clin Microbiol. 2006 Aug;44(8):3025-7.

Evaluation of the GenoType Mycobacteria Direct assay for detection of Mycobacterium tuberculosis complex and four atypical mycobacterial species in clinical samples.

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1
Microbiology Service, Reina Sofia University Hospital, and Mycobacteria Reference Center, Faculty of Medicine, Cordoba University, Cordoba, Spain.

Abstract

We evaluated GenoType Mycobacteria Direct (GTMD), a novel commercial assay based on nucleic acid sequence-based amplification technology, for the detection of Mycobacterium tuberculosis complex, M. avium, M. intracellulare, M. kansasii, and M. malmoense directly from clinical specimens. A total of 134 respiratory and extrapulmonary samples from 65 patients were processed. Sensitivity, specificity, positive predictive, and negative predictive values for GTMD were 92, 100, 100, and 77%, respectively. The GMTD technique is useful, reliable, and rapid when used during the normal routine of a clinical laboratory.

PMID:
16891533
PMCID:
PMC1594618
DOI:
10.1128/JCM.00068-06
[Indexed for MEDLINE]
Free PMC Article
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