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Traffic. 2006 Oct;7(10):1304-10. Epub 2006 Aug 2.

Fast and precise protein tracking using repeated reversible photoactivation.

Author information

1
Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, Moscow 117997, Russia Evrogen JSC, Miklukho-Maklaya 16/10, Moscow 117997, Russia. chudakovdm@mail.ru

Abstract

Photoactivatable fluorescent proteins opened principally novel possibilities to study proteins' movement pathways. In particular, reversibly photoactivatable proteins enable multiple tracking experiments in a long-drawn work with a single cell. Here we report 'protein rivers tracking' technique based on repeated identical rounds of photoactivation and subsequent images averaging, which results in dramatic increase of imaging resolution for fast protein movement events.

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