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J Clin Virol. 2006 Nov;37(3):151-5. Epub 2006 Aug 1.

Comparison of multiple shell vial cell lines for isolation of enteroviruses: a national perspective.

Author information

1
Department of Pathology, University of Utah School of Medicine, 50 N. Medical Drive, Salt Lake City, UT 84132, United States. rosemary.she-bender@hsc.utah.edu

Abstract

BACKGROUND:

Isolation of enterovirus in cell culture is still utilized by clinical laboratories, for vaccine research, and for identifying serotypes for disease surveillance. Use of a combination of cell lines is recommended yet this practice has not been rigorously examined for shell vials.

OBJECTIVES:

To evaluate the growth predilection of enterovirus serotypes for certain shell vial cell lines in clinical samples received at a national reference laboratory.

STUDY DESIGN:

We retrospectively reviewed results of samples submitted for viral culture over a 3-year period. PMK, BGM, RD, A549, and MRC-5 cell lines grown in shell vials were inoculated and serotyped.

RESULTS:

Of 55,816 cultures, 1047 (1.9%) yielded enterovirus representing 18 serotypes. Echovirus 7, echovirus 9, and echovirus 30 were the most common serotypes recovered in 2002, 2003, and 2004, respectively. PMK and MRC-5 recovered the majority of enterovirus isolates; the addition of BGM and RD cells increased our recovery rate by 13%. For 52.6% of enteroviral isolates, cytopathic effect was found in only a single cell line. PMK and BGM cells were effective in isolating coxsackieviruses, and RD and MRC-5 were useful particularly in isolating echoviruses.

CONCLUSIONS:

A combination of shell vial cell lines is still recommended for recovery of enteroviruses.

PMID:
16884950
DOI:
10.1016/j.jcv.2006.06.009
[Indexed for MEDLINE]

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