Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2006;331:267-83.

Microarray approach to identify the signaling network responsible for self-renewal of human embryonic stem cells.

Author information

1
Laboratory of Molecular Embryology, The Rockefeller University, New York, NY, USA.

Abstract

This chapter introduces the representative method to culture human embryonic stem cells (hESCs) under the feeder and feeder-free conditions, the former of which is used to maintain or expand undifferentiated hESCs, and the latter can be used for preparation of pure hESCs RNA samples, or for screening factors influential on self-renewal of hESCs. Also described in this chapter: a protocol and tips for conducting gene chip analysis focusing on widely used Affymetrix microarrays. These techniques will provide an unprecedented scale of biological information to illuminate a key to decipher complex signaling networks controlling pluripotency.

PMID:
16881522
DOI:
10.1385/1-59745-046-4:267
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Support Center