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Methods Mol Biol. 2006;331:115-28.

Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor.

Author information

1
Laboratory of Molecular Embryology, The Rockefeller University, New York, NY, USA.

Abstract

This chapter introduces a new method of maintaining human embryonic stem cells (hESCs) in the undifferentiated state through treatment with a GSK-3 inhibitor, BIO, under a feeder-free condition. Additionally, methods are introduced that determine multidifferentiation potential of hESCs by differentiating into a specific type or random heterogenous cell populations in vitro. These approaches will become a fundamental platform to identify molecular networks regulating cell fate determination in hESCs and mouse embryonic stem cells.

PMID:
16881513
DOI:
10.1385/1-59745-046-4:115
[Indexed for MEDLINE]

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