Format

Send to

Choose Destination
Clin Chem Lab Med. 2006;44(8):987-90.

A robust liquid chromatography tandem mass spectrometry method for total plasma homocysteine determination in clinical practice.

Author information

1
Département de Biologie Intégrée, Centre Hospitalier Universitaire, Grenoble, France. vducros@chu-grenoble.fr

Abstract

BACKGROUND:

Total plasma homocysteine has emerged as an independent risk factor for vascular disease. To meet increasing requests by clinicians for this homocysteine determination, a rapid assay for a routine use has been developed.

METHODS:

A robust, stable, isotope-dilution liquid chromatography tandem mass spectrometry (LC/MS/MS) method is described, including all the practical details and analytical performance results.

RESULTS:

The method allows homocysteine quantitation over a linear working range up to 100 micromol/L, with the limit of quantification estimated at a low value of 0.09 micromol/L. Total analytical imprecision is less than 4%. Accuracy was assessed by measuring the homocysteine concentration in a serum Standard Reference Material.

CONCLUSIONS:

The method was demonstrated to be quick, reliable and cheap after 1 year of use on a time-shared instrument in our hospital unit.

PMID:
16879066
DOI:
10.1515/CCLM.2006.178
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Sheridan PubFactory
Loading ...
Support Center