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J Microbiol Methods. 2006 Dec;67(3):611-5. Epub 2006 Jul 28.

Factors affecting iron sulfide-enhanced bacteriophage plaque assays in Salmonella.

Author information

1
United States Department of Agriculture, Agricultural Research Service, Waste Management and Forage Research Unit, Mississippi State, Mississippi 39762-5367, USA. mmclaughlin@ars.usda.gov <mmclaughlin@ars.usda.gov>

Erratum in

  • J Microbiol Methods. 2007 Feb;68(2):442-3.

Abstract

Reaction of ferric ions with hydrogen sulfide (H(2)S) enhances contrast of phage plaques in H(2)S+ Salmonella, but contrast diminishes in weak H(2)S+ strains. H(2)S was affected by concentrations of peptones, glucose, ferric ammonium citrate (FAC) and sodium thiosulfate (ST), and by FAC:ST ratio, temperature, pH, air, and host strain. Increasing peptone levels was most important for improving contrast in weak H(2)S+ strains.

PMID:
16876271
DOI:
10.1016/j.mimet.2006.05.015
[Indexed for MEDLINE]

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