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Biotechnol Appl Biochem. 2006 Nov;45(Pt 3):167-72.

Recombinant-protein production in insect cells utilizing a hollow-fibre bioreactor.

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Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 11102 New College Building, 245 N. 15th Street, Philadelphia, PA 19102, USA.


Here, we demonstrate for the first time that the hollow-fibre bioreactor is an excellent tool for the production of Drosophila-expressed recombinant proteins. Using the example of the soluble extracellular portion of the human IL-5 (interleukin 5) receptor alpha expression in S2 (Schneider's Drosophila melanogaster cell line 2) cells, we found that it is possible to produce multi-milligram amounts of functional recombinant protein continuously for several months on a laboratory scale with minimal maintenance requirements. The insect cells grow to high density and express concentrated functional recombinant protein in a small volume, simplifying and economizing downstream purification.

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