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Biotechnol Appl Biochem. 2006 Nov;45(Pt 3):167-72.

Recombinant-protein production in insect cells utilizing a hollow-fibre bioreactor.

Author information

1
Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 11102 New College Building, 245 N. 15th Street, Philadelphia, PA 19102, USA. scb28@drexel.edu

Abstract

Here, we demonstrate for the first time that the hollow-fibre bioreactor is an excellent tool for the production of Drosophila-expressed recombinant proteins. Using the example of the soluble extracellular portion of the human IL-5 (interleukin 5) receptor alpha expression in S2 (Schneider's Drosophila melanogaster cell line 2) cells, we found that it is possible to produce multi-milligram amounts of functional recombinant protein continuously for several months on a laboratory scale with minimal maintenance requirements. The insect cells grow to high density and express concentrated functional recombinant protein in a small volume, simplifying and economizing downstream purification.

PMID:
16872272
DOI:
10.1042/BA20060074
[Indexed for MEDLINE]

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