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EMBO J. 2006 Aug 9;25(15):3693-701. Epub 2006 Jul 20.

An essential complementary role of NF-kappaB pathway to microbicidal oxidants in Drosophila gut immunity.

Author information

1
Division of Molecular Life Science and National Creative Research Initiative Center for Symbiosystem, Ewha Woman's University, Seoul, South Korea.

Abstract

In the Drosophila gut, reactive oxygen species (ROS)-dependent immunity is critical to host survival. This is in contrast to the NF-kappaB pathway whose physiological function in the microbe-laden epithelia has yet to be convincingly demonstrated despite playing a critical role during systemic infections. We used a novel in vivo approach to reveal the physiological role of gut NF-kappaB/antimicrobial peptide (AMP) system, which has been 'masked' in the presence of the dominant intestinal ROS-dependent immunity. When fed with ROS-resistant microbes, NF-kappaB pathway mutant flies, but not wild-type flies, become highly susceptible to gut infection. This high lethality can be significantly reduced by either re-introducing Relish expression to Relish mutants or by constitutively expressing a single AMP to the NF-kappaB pathway mutants in the intestine. These results imply that the local 'NF-kappaB/AMP' system acts as an essential 'fail-safe' system, complementary to the ROS-dependent gut immunity, during gut infection with ROS-resistant pathogens. This system provides the Drosophila gut immunity the versatility necessary to manage sporadic invasion of virulent pathogens that somehow counteract or evade the ROS-dependent immunity.

PMID:
16858400
PMCID:
PMC1538556
DOI:
10.1038/sj.emboj.7601233
[Indexed for MEDLINE]
Free PMC Article

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