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J Phys Chem B. 2005 Sep 1;109(34):16511-4.

Hofmeister effects in biology: effect of choline addition on the salt-induced super activity of horseradish peroxidase and its implication for salt resistance of plants.

Author information

1
Dipartimento di Scienze Chimiche, Università di Cagliari-CSGI, Cittadella Monserrato, S.S. 554 Bivio Sestu, 09042 Monserrato-Cagliari, Italy.

Abstract

The effect of choline addition on the salt-induced super activity of horseradish peroxidase (HRP) is investigated. HRP is presented in the literature as an efficient H(2)O(2) scavenger, and choline is the precursor of glycine betaine, a strong osmoprotectant molecule. Both the regulations of H(2)O(2) and of osmoprotectant concentrations are implicated in plants in order to counteract salt-induced cell damage. For the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), sulfate anions were found to play a crucial role in the increase of HRP activity. This induced super activity can be strongly reduced by adding choline chloride. The phenomena provide an example of physicochemical Hofmeister effects playing a central regulatory role in an important biological system.

PMID:
16853099
DOI:
10.1021/jp051897v
[Indexed for MEDLINE]

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