Abstract
Oct-1 is a transcription factor involved in the cell cycle regulation of histone H2B gene transcription and in the transcription of other cellular housekeeping genes. Oct-1 is hyperphosphorylated as cells enter mitosis, and mitosis-specific phosphorylation is reversed as cells exit mitosis. A mitosis-specific phosphorylation site in the homeodomain of Oct-1 was phosphorylated in vitro by protein kinase A. Phosphorylation of this site correlated with inhibition of Oct-1 DNA binding activity in vivo and in vitro. The inhibition of Oct-1 DNA binding during mitosis suggests a mechanism by which the general inhibition of transcription during mitosis might occur.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cattle
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Cell Cycle
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Cloning, Molecular
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DNA, Neoplasm / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Genes, Homeobox
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HeLa Cells
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Histones / genetics
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Host Cell Factor C1
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Humans
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Mitosis
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Molecular Sequence Data
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Myocardium / enzymology
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Octamer Transcription Factor-1
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Oligodeoxyribonucleotides
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Peptide Mapping
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Phosphopeptides / isolation & purification
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Phosphorylation
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Protein Kinases / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism*
Substances
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DNA, Neoplasm
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DNA-Binding Proteins
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HCFC1 protein, human
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Histones
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Host Cell Factor C1
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Octamer Transcription Factor-1
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Oligodeoxyribonucleotides
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POU2F1 protein, human
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Phosphopeptides
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Transcription Factors
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Protein Kinases