DNA damage response and mutagenesis in mouse embryonic stem cells

Yiling Hong; Rachel B Cervantes; Peter J Stambrook

doi:10.1385/1-59745-037-5:313

DNA damage response and mutagenesis in mouse embryonic stem cells

Methods Mol Biol. 2006:329:313-26. doi: 10.1385/1-59745-037-5:313.

Authors

Yiling Hong¹, Rachel B Cervantes, Peter J Stambrook

Affiliation

¹ Department of Cell Biology, Neurobiology and Anatomy, University of Cincinnati College of Medicine, OH, USA.

PMID: 16846001
DOI: 10.1385/1-59745-037-5:313

Abstract

Mutation in embryonic stem (ES) cells can potentially compromise multiple cell lineages and affect the well-being of subsequent generations. Thus, ES cells require sensitive mechanisms to maintain genomic integrity. One mechanism involves suppression of mutation. A complementary mechanism is to regulate the cell cycle checkpoint and facilitate cell death. Here, we describe the detailed protocols we have used to investigate DNA damage response and mutagenesis in mouse ES cells.

MeSH terms

Adenine Phosphoribosyltransferase / deficiency
Adenine Phosphoribosyltransferase / genetics
Animals
Apoptosis
Base Sequence
Cell Culture Techniques / methods
Cell Cycle
Coculture Techniques
Colony-Forming Units Assay
DNA / genetics
DNA Damage*
Embryo, Mammalian / cytology*
Female
Gene Targeting
Genetic Vectors
Genomic Instability
Mice
Mice, Inbred C3H
Mice, Knockout
Mutagenesis*
Pluripotent Stem Cells / cytology
Pluripotent Stem Cells / metabolism*
Pregnancy

Substances

DNA
Adenine Phosphoribosyltransferase