Format

Send to

Choose Destination
J Immunol Methods. 2006 Jul 31;314(1-2):164-9. Epub 2006 Jun 23.

Soluble Dectin-1 as a tool to detect beta-glucans.

Author information

1
Institute of Infectious Disease and Molecular Medicine, CLS, Faculty of Health Sciences, University of Cape Town, Observatory, 7925 Cape Town, South Africa.

Abstract

Beta-glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian beta-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble beta-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.

PMID:
16844139
DOI:
10.1016/j.jim.2006.05.013
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center