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Neurosci Lett. 1991 Aug 19;129(2):254-8.

Survival of developing dopaminergic neurons in reaggregate tissue culture following treatment with methamphetamine.

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Department of Pharmacological and Physiological Sciences, University of Chicago, IL 60637.


Developing central dopamine (DA)-containing neurons in rotation-mediated reaggregate tissue culture exposed to 10(-4) M methamphetamine for 1 week (at a time corresponding to the 2nd week of postnatal development) exhibit decreased DA levels. DA neurons were visualized either by a histofluorescent method after 'loading' with exogenous DA, or by tyrosine hydroxylase (TH) immunocytochemistry. An apparent loss of histofluorescent DA cell bodies was observed due to a methamphetamine-induced reduction in exogenous DA accumulation. No decrease in the number of TH-immunoreactive neurons was observed. Thus, developing DA neurons when visualized by TH immunocytochemistry survive despite methamphetamine-induced reductions in DA levels.

[Indexed for MEDLINE]

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