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Nano Lett. 2006 Jul;6(7):1491-5.

Tracking individual kinesin motors in living cells using single quantum-dot imaging.

Author information

1
Laboratoire Kastler Brossel, CNRS UMR 8552, Ecole normale supérieure, Université Pierre et Marie Curie-Paris 6, Paris, France.

Abstract

We report a simple method using semiconductor quantum dots (QDs) to track the motion of intracellular proteins with a high sensitivity. We characterized the in vivo motion of individual QD-tagged kinesin motors in living HeLa cells. Single-molecule measurements provided important parameters of the motor, such as its velocity and processivity, as well as an estimate of the force necessary to carry a QD. Our measurements demonstrate the importance of single-molecule experiments in the investigation of intracellular transport as well as the potential of single quantum-dot imaging for the study of important processes such as cellular trafficking, cell polarization, and division.

PMID:
16834436
DOI:
10.1021/nl060921t
[Indexed for MEDLINE]

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