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J Gen Microbiol. 1991 Jul;137(7):1459-69.

DNA polymorphisms in new isolates of 'Deinococcus radiopugnans'.

Author information

1
Department of Pathology, F. E. H├ębert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799.

Abstract

Nineteen new Deinococcus isolates from soil, 18 of which were from samples immediately adjacent to a lake in Nottingham, UK, were characterized by conventional criteria, and found to be identical to each other and to conform most closely to the species D. radiopugnans. However, we detected three different restriction enzyme activities in three different isolates. Because of this suggestion of heterogeneity, we examined the isolates for restriction fragment length polymorphisms (RFLPs). RFLP analysis of the 19 original isolates, using three different probes, distinguished 17 divergent groups. This extraordinary diversity cannot be attributed to geographical differences, nor to the method of isolation, which employed UV-radiation selection.

PMID:
1683397
DOI:
10.1099/00221287-137-7-1459
[Indexed for MEDLINE]

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