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Opt Lett. 2006 Aug 1;31(15):2305-7.

Three-dimensional optic axis determination using variable-incidence-angle polarization-optical coherence tomography.

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  • 1Biomedical Physics Group, University of Exeter, Stocker Road, Exeter EX4 4QL, UK.


Polarization optical coherence tomography (PSOCT) is a powerful technique to nondestructively map the retardance and fast-axis orientation of birefringent biological tissues. Previous studies have concentrated on the case where the optic axis lies on the plane of the surface. We describe a method to determine the polar angle of the optic axis of a uniaxial birefringent tissue by making PSOCT measurements with a number of incident illumination directions. The method is validated on equine flexor tendon, yielding a variability of 4% for the true birefringence and 3% for the polar angle. We use the method to map the polar angle of fibers in the transitional region of equine cartilage.

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