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J Cell Biol. 2006 Jul 17;174(2):231-43. Epub 2006 Jul 10.

Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration ability.

Author information

1
Stem Cell Research Institute, San Raffaele Hospital, 20132 Milan, and Department of Experimental Medicine, Human Anatomy Institute, University of Pavia, Italy.

Erratum in

  • J Cell Biol. 2006 Aug 14;174(4):605.
  • J Cell Biol. 2006 Oct 23;175(2):361. Costantin, Gabriela [corrected to Constantin, Gabriela].
  • J Cell Biol. 2013 Aug 19;202(4):715-6.

Abstract

Efficient delivery of cells to target tissues is a major problem in cell therapy. We report that enhancing delivery of mesoangioblasts leads to a complete reconstitution of downstream skeletal muscles in a mouse model of severe muscular dystrophy (alpha-sarcoglycan ko). Mesoangioblasts, vessel-associated stem cells, were exposed to several cytokines, among which stromal- derived factor (SDF) 1 or tumor necrosis factor (TNF) alpha were the most potent in enhancing transmigration in vitro and migration into dystrophic muscle in vivo. Transient expression of alpha4 integrins or L-selectin also increased several fold migration both in vitro and in vivo. Therefore, combined pretreatment with SDF-1 or TNF-alpha and expression of alpha4 integrin leads to massive colonization (>50%) followed by reconstitution of >80% of alpha-sarcoglycan-expressing fibers, with a fivefold increase in efficiency in comparison with control cells. This study defines the requirements for efficient engraftment of mesoangioblasts and offers a new potent tool to optimize future cell therapy protocols for muscular dystrophies.

PMID:
16831885
PMCID:
PMC2064183
DOI:
10.1083/jcb.200512085
[Indexed for MEDLINE]
Free PMC Article
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