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Curr Opin Biotechnol. 2006 Aug;17(4):373-80. Epub 2006 Jul 7.

Cell-free expression systems for eukaryotic protein production.

Author information

  • 1Cell-Free Science and Technology Research Center, Ehime University, Matsuyama, Japan. yendo@eng.ehime-u.ac.jp

Abstract

Following the success of genome sequencing projects, attention has now turned to studies of the structure and function of proteins. Although cell-based expression systems for protein production have been widely used, they have certain limitations in terms of the quality and quantity of the proteins produced and for high-throughput production. Many of these limitations can be circumvented by the use of cell-free translation systems. Among such systems, the wheat germ based system is of special interest for its eukaryotic nature; it has the significant advantage of producing eukaryotic multidomain proteins in a folded state. Several advances in the use of cell-free expression systems have been made in the past few years and successful applications of these systems to produce proteins for functional and structural biology studies have been reported.

PMID:
16828277
DOI:
10.1016/j.copbio.2006.06.009
[PubMed - indexed for MEDLINE]
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