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Nat Biotechnol. 2006 Jul;24(7):820-1. Epub 2006 Jun 25.

Transposon-free insertions for insect genetic engineering.

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1
Oxitec Limited, 71 Milton Park, Oxford OX14 4RX, UK.

Abstract

Methods involving the release of transgenic insects in the field hold great promise for controlling vector-borne diseases and agricultural pests. Insect transformation depends on nonautonomous transposable elements as gene vectors. The resulting insertions are stable in the absence of suitable transposase, however, such absence cannot always be guaranteed. We describe a method for post-integration elimination of all transposon sequences in the pest insect Medfly, Ceratitis capitata. The resulting insertions lack transposon sequences and are therefore impervious to transposase activity.

PMID:
16823373
DOI:
10.1038/nbt1221
[Indexed for MEDLINE]
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