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Virology. 2006 Sep 30;353(2):344-56. Epub 2006 Jul 3.

Rabies virus glycoprotein as a carrier for anthrax protective antigen.

Author information

1
Department of Microbiology, Thomas Jefferson University, Philadelphia, PA 19107, USA.

Abstract

Live viral vectors expressing foreign antigens have shown great promise as vaccines against viral diseases. However, safety concerns remain a major problem regarding the use of even highly attenuated viral vectors. Using the rabies virus (RV) envelope protein as a carrier molecule, we show here that inactivated RV particles can be utilized to present Bacillus anthracis protective antigen (PA) domain-4 in the viral membrane. In addition to the RV glycoprotein (G) transmembrane and cytoplasmic domains, a portion of the RV G ectodomain was required to express the chimeric RV G anthrax PA on the cell surface. The novel antigen was also efficiently incorporated into RV virions. Mice immunized with the inactivated recombinant RV virions exhibited seroconversion against both RV G and anthrax PA, and a second inoculation greatly increased these responses. These data demonstrate that a viral envelope protein can carry a bacterial protein and that a viral carrier can display whole polypeptides compared to the limited epitope presentation of previous viral systems.

PMID:
16820183
PMCID:
PMC1576297
DOI:
10.1016/j.virol.2006.05.010
[Indexed for MEDLINE]
Free PMC Article

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