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Genes Dev. 2006 Jul 1;20(13):1721-6.

BRCA1 ubiquitinates its phosphorylation-dependent binding partner CtIP.

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1
Department of Oncology, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.

Abstract

BRCA1 (Breast Cancer Susceptibility Gene 1) possesses an N-terminal Ring domain and tandem C-terminal BRCT motifs. While the Ring domain has E3 ubiquitin ligase activity, the BRCA1 BRCT domains specifically recognize phospho-serine motifs. Here, we demonstrate that BRCA1 Ring domain catalyzes CtIP ubiquitination in a manner that depends on a phosphorylation-mediated interaction between CtIP and BRCA1 BRCT domains. The BRCA1-dependent ubiquitination of CtIP does not target CtIP for degradation. Instead, ubiquitinated CtIP associates with chromatin following DNA damage and participates in G2/M checkpoint control. Thus, we propose that BRCA1 can regulate the functions of its substrates through nonproteasomal pathways that do not involve substrate degradation.

PMID:
16818604
PMCID:
PMC1522068
DOI:
10.1101/gad.1431006
[Indexed for MEDLINE]
Free PMC Article
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