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J Allergy Clin Immunol. 2006 Jul;118(1):91-7. Epub 2006 Apr 27.

The course of allergen-induced leukocyte infiltration in human and experimental asthma.

Author information

1
Department of Pneumology, Clinic of Internal Medicine, University of Rostock, Ernst-Heydemann-Strasse 6, 18057 Rostock, Germany. marek.lommatzsch@med.uni-rostock.de

Abstract

BACKGROUND:

Although the timing of allergen-induced bronchoconstriction is well defined, there is little information about the kinetics of allergen-induced leukocyte infiltration in asthma and its comparability between human and animal models of asthma.

OBJECTIVE:

To investigate systematically allergen-induced leukocyte infiltration into the airway lumen in human and experimental asthma by using bronchoalveolar lavage.

METHODS:

Patients with allergic asthma were lavaged at different time points as long as 1 week after segmental allergen challenge. Allergen-sensitized mice were lavaged as long as 3 weeks after allergen challenge. Differential cell counts, lymphocyte subsets, and cytokines were assessed in bronchoalveolar lavage fluid.

RESULTS:

In both models, neutrophil infiltration was a relatively early event (maximum: 18 hours after challenge). In contrast, eosinophil infiltration peaked 42 hours (human model) to 4 days (mouse model) after allergen challenge, paralleled by an IL-5 peak in this period. There were elevated macrophage counts over a period of several days after allergen challenge in both models. Lymphocytes (predominantly CD4+ T cells) peaked 18 hours after challenge in the human model, but not until 2 weeks after challenge in the murine model.

CONCLUSION:

Early neutrophil accumulation (within hours after challenge) and delayed eosinophil accumulation (within days after challenge) in the airway lumen are common features of allergen-induced airway inflammation, whereas lymphocyte kinetics are dependent on the asthma model.

CLINICAL IMPLICATIONS:

Similarities in the infiltration kinetics of granulocytes after allergen challenge suggest a common role for these cells in asthma, whereas the presumed orchestration of allergic inflammation by lymphocytes appears to differ between the models.

PMID:
16815143
DOI:
10.1016/j.jaci.2006.02.034
[Indexed for MEDLINE]

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