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Clin Biochem. 2006 Aug;39(8):826-32. Epub 2006 May 19.

Novel comparison of capillary electrophoresis versus immunoassay in the measurement of total percent carbohydrate deficient transferrin.

Author information

1
Department of Epidemiology and Preventive Medicine, University of Maryland, Baltimore, Baltimore, MD 21201, USA. kstrauss@umaryland.edu

Abstract

OBJECTIVES:

Novel comparison of CDT isoforms as determined by CE with an FDA-approved immunoassay kit.

DESIGN AND METHODS:

Subjects (n=51) were categorized by drinking status based on AUDIT questionnaire responses. CDT isoform analyses by CE were compared to a commercially available, FDA-approved immunoassay. The analytical specificity of the immunoassay kit was assessed by analysis with IEF.

RESULTS:

Because of the poor correlation between % CDT as measured by CE and the TIA immunoassay and between subject-reported drinking levels and results from the TIA assay, extraction column eluants from the immunoassays were analyzed by IEF for analytical specificity. % CDT by TIA included some trisialo-Tf, a non-CDT fraction, in the % CDT determination.

CONCLUSIONS:

Total % CDT by CE, which separates all isoforms is more analytically specific than immunoassays because it does not include trisialo-Tf in the CDT calculation.

[Indexed for MEDLINE]

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