Send to

Choose Destination
Clin Biochem. 2006 Aug;39(8):826-32. Epub 2006 May 19.

Novel comparison of capillary electrophoresis versus immunoassay in the measurement of total percent carbohydrate deficient transferrin.

Author information

Department of Epidemiology and Preventive Medicine, University of Maryland, Baltimore, Baltimore, MD 21201, USA.



Novel comparison of CDT isoforms as determined by CE with an FDA-approved immunoassay kit.


Subjects (n=51) were categorized by drinking status based on AUDIT questionnaire responses. CDT isoform analyses by CE were compared to a commercially available, FDA-approved immunoassay. The analytical specificity of the immunoassay kit was assessed by analysis with IEF.


Because of the poor correlation between % CDT as measured by CE and the TIA immunoassay and between subject-reported drinking levels and results from the TIA assay, extraction column eluants from the immunoassays were analyzed by IEF for analytical specificity. % CDT by TIA included some trisialo-Tf, a non-CDT fraction, in the % CDT determination.


Total % CDT by CE, which separates all isoforms is more analytically specific than immunoassays because it does not include trisialo-Tf in the CDT calculation.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center