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EMBO Rep. 2006 Jul;7(7):710-5. Epub 2006 Jun 16.

Homeostatic regulation of supercoiling sensitivity coordinates transcription of the bacterial genome.

Author information

1
International University Bremen, Campus Ring 1, 28759 Bremen, Germany.

Abstract

Regulation of cellular growth implies spatiotemporally coordinated programmes of gene transcription. A central question, therefore, is how global transcription is coordinated in the genome. The growth of the unicellular organism Escherichia coli is associated with changes in both the global superhelicity modulated by cellular topoisomerase activity and the relative proportions of the abundant DNA-architectural chromatin proteins. Using a DNA-microarray-based approach that combines mutations in the genes of two important chromatin proteins with induced changes of DNA superhelicity, we demonstrate that genomic transcription is tightly associated with the spatial distribution of supercoiling sensitivity, which in turn depends on chromatin proteins. We further demonstrate that essential metabolic pathways involved in the maintenance of growth respond distinctly to changes of superhelicity. We infer that a homeostatic mechanism organizing the supercoiling sensitivity is coordinating the growth-phase-dependent transcription of the genome.

PMID:
16799466
PMCID:
PMC1500834
DOI:
10.1038/sj.embor.7400729
[Indexed for MEDLINE]
Free PMC Article
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