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Methods Enzymol. 2006;408:264-73.

An assay for studying ubiquitylation of RNA polymerase II and other proteins in crude yeast extracts.

Author information

1
Cancer Research United Kingdom, London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire.

Abstract

In recent years, much research effort has been invested in understanding the role and importance of protein ubiquitylation. This chapter presents protocols that enable ubiquitylation of RNAPII and other proteins to be reconstituted in crude yeast extracts in vitro. These procedures, combined with the ease of genetic manipulations in yeast, provide a new powerful tool for investigating the mechanisms underlying protein ubiquitylation.

PMID:
16793374
DOI:
10.1016/S0076-6879(06)08016-5
[Indexed for MEDLINE]

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